LOCUS       EF1a-FLuc-F2A-GF        8145 bp    DNA     linear        8-JUL-2013
SOURCE      
  ORGANISM  
            This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     LTR             7911..8145
                     /label=3'\LTR
     intron          291..1455
                     /label=Intron
     LTR             1..181
                     /label=5'LTR
     misc_feature    7232..7828
                     /label=WPRE
     promoter        1698..3087
                     /label=human\EF1a\promoter
     intron          2126..3068
                     /label=intron
     CDS             6197..7222
                     /label=Hyg
     RBS             5589..6173
                     /label=IRES
     CDS             4799..4864
                     /label=F2A
     CDS             4865..5584
                     /label=GFP
     CDS             3134..4783
                     /label=Firefly\Luciferase\(FLuc)
BASE COUNT     1923 a      2177 c      2299 g      1746 t 
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 
     1681 ttcaaaattt tatcgatgcc tccccgtcac cacccccccc aacccgcccc gaccggagct 
     1741 gagagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta 
     1801 aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc 
     1861 tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga 
     1921 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 
     1981 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 
     2041 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 
     2101 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 
     2161 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg 
     2221 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 
     2281 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 
     2341 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 
     2401 aaaatttttg atgatatcct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 
     2461 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 
     2521 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 
     2581 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg 
     2641 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 
     2701 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 
     2761 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 
     2821 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 
     2881 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 
     2941 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 
     3001 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 
     3061 catttcaggt gtcgtgaaaa ctacccctct agataatacg actcactata gggcccgggt 
     3121 tggatccgcc accatggaag atgccaaaaa cattaagaag ggcccagcgc cattctaccc 
     3181 actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct acgccctggt 
     3241 gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct acgccgagta 
     3301 cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga atacaaacca 
     3361 tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt tgggtgccct 
     3421 gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg agctgctgaa 
     3481 cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat 
     3541 cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg atagcaagac 
     3601 cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc cacccggctt 
     3661 caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg ccctgatcat 
     3721 gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg 
     3781 tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc ccgacaccgc 
     3841 tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc tgggctactt 
     3901 gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat tcttgcgcag 
     3961 cttgcaagac tataagattc aatctgccct gctggtgccc acactattta gcttcttcgc 
     4021 taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg ccagcggcgg 
     4081 ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc taccaggcat 
     4141 ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc ccgaagggga 
     4201 cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg tggtggactt 
     4261 ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat 
     4321 gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg acaaggacgg 
     4381 ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct tcatcgtgga 
     4441 ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg aactggagag 
     4501 catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc ccgacgacga 
     4561 tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa 
     4621 ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt 
     4681 tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc gcaagatccg 
     4741 cgagattctc attaaggcca agaagggcgg caagatcgcc gtggctagcg gcgatatcgt 
     4801 gaagcagacc ctgaacttcg acctcctgaa gctggccggc gacgtggaaa gcaaccccgg 
     4861 gcctatggtg tccaagggcg aggaactgtt caccggcgtg gtgcccatcc tggtggaact 
     4921 ggatggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgaag gcgacgccac 
     4981 atatggcaag ctgacactga agttcatctg caccaccggc aagctgcccg tgccttggcc 
     5041 taccctcgtg accacactga cctacggcgt gcagtgcttc tccagatacc ccgaccatat 
     5101 gaagcagcac gacttcttca agagcgccat gcccgagggc tacgtgcagg aacggaccat 
     5161 cttctttaag gacgacggca actacaagac cagggccgaa gtgaagttcg agggcgacac 
     5221 cctcgtgaac cggatcgagc tgaagggcat cgacttcaaa gaggacggca acatcctggg 
     5281 ccacaagctg gagtacaact acaacagcca caacgtctac atcatggccg acaagcagaa 
     5341 aaacggcatc aaagtgaact tcaagatccg gcacaacatc gaggacggct ccgtgcagct 
     5401 ggccgaccac taccagcaga acacccccat cggagatggc cccgtgctgc tgcccgacaa 
     5461 ccactacctg agcacacaga gcgccctgag caaggacccc aacgagaagc gggaccacat 
     5521 ggtgctgctg gagttcgtga ccgccgctgg catcaccctg ggcatggacg agctgtacaa 
     5581 gtgaactagc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata 
     5641 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt 
     5701 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct 
     5761 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 
     5821 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga 
     5881 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 
     5941 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 
     6001 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 
     6061 gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc 
     6121 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acactagaga 
     6181 tccaccggtc gccaccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct 
     6241 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg 
     6301 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga 
     6361 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc 
     6421 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc 
     6481 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt 
     6541 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc 
     6601 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc 
     6661 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc 
     6721 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt 
     6781 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat 
     6841 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg 
     6901 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga 
     6961 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta 
     7021 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc 
     7081 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc 
     7141 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac 
     7201 tcgtccgagg gcaaaggaat aggcggccgc gtcgacaatc aacctctgga ttacaaaatt 
     7261 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 
     7321 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 
     7381 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 
     7441 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 
     7501 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 
     7561 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 
     7621 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 
     7681 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 
     7741 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 
     7801 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga 
     7861 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 
     7921 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag 
     7981 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 
     8041 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 
     8101 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagca 
//
